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Culture media consisted of 10 g/L glucose, 1 g/L yeast extract, 5 g/L (NH 4 ) 2 SO 4 , 2 g/L KH 2 PO 4 , 0.5 g/L MgSO 4 ·7H 2 O, 0.1 g/L CaCl 2 ·2H 2 O, 0.02 g/L FeSO 4 ·7H 2 O, 0.01 g/L ZnSO 4 ·7H 2 O, 0.005 g/L CuSO 4 ·5H 2 O, 0.001 g/L MnCl 2 ·4H 2 O, 2.5 mL of 70% H 2 SO 4 and 0.5% (v/v) Antifoam 204 (Sigma Aldrich, St

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In addition, further studies using cell-based in vitro assays and in vivo models are warranted to evaluate the anti-cholinesterase effects of cannabinoids and to elucidate their mechanism of action